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neurona and using reactivity to the 30- and 16-kD bands as the criterion for a positive test. It is concluded that the specificity of the Western blot test is improved by blocking proteins not specific to S. cruzi antibody-blocked Western blot had a sample sensitivity of 100% and sample specificity of 98%. Reverse transcriptase polymerase chain reaction(PCR) analyses and Western blot indicated that the mRNA and protein levels of EPM in the transgenic cell. but studies that used Western blot to confirm serologic results have. After this modification of the Western blot test, positive infection status was significantly associated with reactivity to the 30- and 16-kD bands (P<0.001, Fisher's exact test). Equine protozoal myeloencephalitis (EPM) remains an important neurologic disease of. cruzi antibodies prior to loading the equine samples. neurona does not exist) were tested by Western blot. Replication of quantitative analysis of Western blot data results confirms the validity and reproducibility of any observed changes 13, 14, 18, 20, 21. neurona infections (confirmed by culture from neural tissue) and 57 horses without infections (horses from the Eastern Hemisphere, where S. Separated proteins were electrophoretically transferred to polyvinylidene fluoride membranes and blocked in 1% bovine serum albumin and 0.5% Tween-Tris-buffered saline. to minimize false-positive results in the western blot test for S. neurona, but will offer a false negative if the disease is caused by N. Sarcocystis neurona merozoites harvested from equine dermal cell culture were heat denatured, and the proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in a 12-20% linear gradient gel. Equine protozoal myeloencephalitis (EPM) is a neurological disease of horses. The Western Blot test identifies EPM cases caused by S. The purposes of this study were to develop the most stringent criteria possible for a positive test result, to estimate the sensitivity and specificity of the EPM Western blot antibody test, and to assess the ability of bovine antibodies to Sarcocystis cruzi to act as a blocking agent to minimize false-positive results in the western blot test for S. Equine protozoal myeloencephalitis (EPM) is a neurological disease of horses and ponies caused by the apicomplexan protozoan parasite Sarcocystis neurona.